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| Line 27: | Line 27: | ||
*[[Bradford Assay]] | *[[Bradford Assay]] | ||
*[[Quantification by Absorbance at 280nm]] | *[[Quantification by Absorbance at 280nm]] | ||
*[[ | *[[Determining Percent Purity]] | ||
===Protein Purification=== | ===Protein Purification=== | ||
*[[French Press]] | *[[French Press]] | ||
*[[Purification of GST Fusion Proteins]] | *[[Purification of GST Fusion Proteins]] | ||
*[[ | *[[Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)]] | ||
==Transcriptional Analysis== | ==Transcriptional Analysis== | ||
Revision as of 19:21, 30 June 2009
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Fugene Transfection of 293T/COS Cells
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
- Luciferase Assay
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
- Cesium Chloride Preparation of DNA
Protein Analysis
Protein Quantification
Protein Purification
- French Press
- Purification of GST Fusion Proteins
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)