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*[[Fugene Transfection of 293T/COS Cells]] | *[[Fugene Transfection of 293T/COS Cells]] | ||
*[[Immunofluoresence]] | *[[Immunofluoresence]] | ||
**[[Colocalization]] | |||
*[[3T3-L1 Adipocyte Fractionation]] | *[[3T3-L1 Adipocyte Fractionation]] | ||
*[[Preparing Cell Lysates]] | *[[Preparing Cell Lysates]] | ||
*[[Glucose Uptake Assay]] | *[[Glucose Uptake Assay]] | ||
*[[Luciferase Assay]] | *[[Luciferase Assay]] | ||
==Cloning and Molecular Biology== | ==Cloning and Molecular Biology== | ||
Revision as of 15:32, 10 June 2009
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Fugene Transfection of 293T/COS Cells
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
- Luciferase Assay
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
- Cesium Chloride Preparation of DNA
Protein Analysis
Protein Quantification
- Bradford Assay
- Quantification by Absorbance at 280nm
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)