PCR Analysis of Tail DNA: Difference between revisions
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==Protocol== | ==Protocol== | ||
Use the following | Use the following Volumes per Reaction: | ||
Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer) | Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer) | ||
Forward Primer: .4ul | Forward Primer: .4ul | ||
Reverse Primer: .4ul | Reverse Primer: .4ul | ||
dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer) | |||
dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer) | |||
Sterile water: 13.6 uL | Sterile water: 13.6 uL | ||
Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer) | Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer) | ||
Template: 1 uL | Template: 1 uL | ||
see [[Preparing an Agarose Gel]] for details on preparing a DNA gel | see [[Preparing an Agarose Gel]] for details on preparing a DNA gel | ||
Revision as of 16:28, 5 June 2009
see Genotyping Details for strain specific details
Materials
Protocol
Use the following Volumes per Reaction:
Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer)
Forward Primer: .4ul
Reverse Primer: .4ul
dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer)
Sterile water: 13.6 uL
Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer)
Template: 1 uL
see Preparing an Agarose Gel for details on preparing a DNA gel