Phosphate Buffered Saline: Difference between revisions

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Created page with "==Recipe== * Start with 800 ml of distilled water: * Add 8 g of NaCl. * Add 0.2 g of KCl. * Add 1.44 g of Na<sub>2</sub>HPO<sub>4</sub>. * Add 0.24 g of KH<sub>2</sub>PO<sub>4..."
 
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==Recipe==
==Recipe==
* Start with 800 ml of distilled water:
* Start with 400ml of distilled water:
* Add 8 g of NaCl.
* Add 4 g of NaCl.
* Add 0.2 g of KCl.
* Add 0.72 g of KCl.
* Add 1.44 g of Na<sub>2</sub>HPO<sub>4</sub>.
* Add 1.72 g of Na<sub>2</sub>HPO<sub>4</sub>.
* Add 0.24 g of KH<sub>2</sub>PO<sub>4</sub>.
* Add 0.12 g of KH<sub>2</sub>PO<sub>4</sub>.
* Adjust the pH to 7.4 with HCl.
* Adjust the pH to 7.4 with HCl.
* Add distilled water to a total volume of 1 liter.
* Add distilled water to a total volume of 500 mL.

Revision as of 21:54, 25 January 2018

Recipe

  • Start with 400ml of distilled water:
  • Add 4 g of NaCl.
  • Add 0.72 g of KCl.
  • Add 1.72 g of Na2HPO4.
  • Add 0.12 g of KH2PO4.
  • Adjust the pH to 7.4 with HCl.
  • Add distilled water to a total volume of 500 mL.