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*[[DNA Preparation from Tail Clip]] | *[[DNA Preparation from Tail Clip]] | ||
*[[PCR Analysis of Tail DNA]] | *[[PCR Analysis of Tail DNA]] | ||
==Metabolic Measurements== | ===Metabolic Measurements=== | ||
*[[Glucose Tolerance Test]] | *[[Glucose Tolerance Test]] | ||
Revision as of 13:43, 13 May 2009
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Harvesting RNA from Cells grown in monolayer
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel