Cre PCR: Difference between revisions
Jump to navigation
Jump to search
copied in PCR program |
added primers |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==PCR Program== | ==PCR Program== | ||
* Cre (F) GCATT ACCGG GCAAC GAGTG ATGAG | |||
* Cre (R) GAGTG AACGA ACCTG GTCGA AATCA GTGCG | |||
*95C 1min | *95C 1min | ||
* | *35 Cycles of: | ||
**95C 10s | **95C 10s | ||
**55C 30s | **55C 30s | ||
| Line 8: | Line 11: | ||
*72C 5min | *72C 5min | ||
*4C forever | *4C forever | ||
[[ Category:Genotyping ]] | |||
[[ Category:Mouse Work]] | |||
[[ Category:PCR]] | |||
[[ Category:DNA]] | |||
[[ Category:Molecular Biology]] | |||
Latest revision as of 18:42, 25 April 2018
PCR Program
- Cre (F) GCATT ACCGG GCAAC GAGTG ATGAG
- Cre (R) GAGTG AACGA ACCTG GTCGA AATCA GTGCG
- 95C 1min
- 35 Cycles of:
- 95C 10s
- 55C 30s
- 72C 45s
- 72C 5min
- 4C forever