Measuring Cross-Sectional Area (CSA): Difference between revisions
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#Use the Scrolling tool(hand) to grab and move the picture | #Use the Scrolling tool(hand) to grab and move the picture | ||
#Set the scale with the *Straight* (line tool). Click on one end of the scale and hold shift to keep the line straight to the other end | #Set the scale with the *Straight* (line tool). Click on one end of the scale and hold shift to keep the line straight to the other end | ||
#"Analyze" | |||
#"Set scale" | |||
#Analyze | #"Known distance:" 200 (for 20x) | ||
#Set scale | #"Unit of length:" um | ||
#Known distance: 200 (for 20x) | #"OK" | ||
#Unit of length: um | |||
#OK | |||
#Adjust the zoom. Start in upper left hand corner | #Adjust the zoom. Start in upper left hand corner | ||
#Use the Freehand selections tool (bean) to trace the fiber by holding down mouse press M | #Use the Freehand selections tool (bean) to trace the fiber by holding down mouse press "M" | ||
#Continue tracing and press M after each fiber trace | #Continue tracing and press "M" after each fiber trace | ||
#Results | #"Results" | ||
#Summarize | #"Summarize" | ||
Latest revision as of 18:43, 27 August 2018
Using ImageJ
- Drag your image into ImageJ.
- Use the Magnifying glass. Click on the image to zoom in and right click to zoom out
- Zoom in on the scale on your image
- Use the Scrolling tool(hand) to grab and move the picture
- Set the scale with the *Straight* (line tool). Click on one end of the scale and hold shift to keep the line straight to the other end
- "Analyze"
- "Set scale"
- "Known distance:" 200 (for 20x)
- "Unit of length:" um
- "OK"
- Adjust the zoom. Start in upper left hand corner
- Use the Freehand selections tool (bean) to trace the fiber by holding down mouse press "M"
- Continue tracing and press "M" after each fiber trace
- "Results"
- "Summarize"