Glucose Tolerance Test: Difference between revisions
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==Materials (Bring | ==SOP== | ||
*Glucometer | *[[SOP - Needle Capping]] | ||
*Glucose Test Strips | *[[Safety and Animal Training]] | ||
==Materials (Bring to Procedure Room)== | |||
*Glucometer - AccuChek Advantage | |||
*Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]]. collect in university hospital building, floor B2 in room B2F408 dock 5 across from central pharmacy. | |||
*Scale | *Scale | ||
*Beaker for weighing mice | *Beaker for weighing mice | ||
*Syringes | *Syringes | ||
*10% Glucose in PBS (sterile filtered). This will correspond to 1g/kg glucose injections. | *10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections. | ||
*If doing an oral glucose tolerance test need a gavage needle | |||
*Timer | |||
==Protocol== | ==Protocol== | ||
#Remove food from mice for about 6h. Add do not feed tag to cages. | #Remove food from mice for about 6h. Add do not feed tag to cages. | ||
#Bring mice to procedure room. | #Bring mice to procedure room. Put water bottle on cage. | ||
#Weigh mice, mark tails as necessary and take fasting glucose measurement. | #Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capillary about 2/3 full with blood. | ||
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | #At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse or the mouth if doing an oral glucose tolerance test. | ||
##Immobilize mouse and restrain tail with one hand | |||
##'''For ipGTT''' Aim needle between the midline and the hip bone | |||
##'''For oGTT''' Gently inert gavage needle into mouth | |||
: | ##Insert syringe (do not inject) into cavity/mouth | ||
##'''For ipGTT:''' Pull up the syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over | |||
##Eject syringe. | |||
#At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein | |||
[[Category:Animal Work]] | |||
[[Category:Mouse Work]] | |||
[[Category:Glucose Homeostasis]] | |||
[[Category:Injections]] | |||
Latest revision as of 16:42, 10 February 2025
SOP
Materials (Bring to Procedure Room)
- Glucometer - AccuChek Advantage
- Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [here]. collect in university hospital building, floor B2 in room B2F408 dock 5 across from central pharmacy.
- Scale
- Beaker for weighing mice
- Syringes
- 10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
- If doing an oral glucose tolerance test need a gavage needle
- Timer
Protocol
- Remove food from mice for about 6h. Add do not feed tag to cages.
- Bring mice to procedure room. Put water bottle on cage.
- Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for Measuring Fasting Insulin by filling a heparinized capillary about 2/3 full with blood.
- Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
- At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse or the mouth if doing an oral glucose tolerance test.
- Immobilize mouse and restrain tail with one hand
- For ipGTT Aim needle between the midline and the hip bone
- For oGTT Gently inert gavage needle into mouth
- Insert syringe (do not inject) into cavity/mouth
- For ipGTT: Pull up the syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
- Eject syringe.
- At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein