Measuring Fasting Insulin: Difference between revisions

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==Materials==
==Materials==
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
*Heparinzed capilaries


==Protocol==
==Protocol==
#Collect about 2/3 of a capillary of blood.
#Collect serum (see [[Collecting_and_Storing_Mouse_Serum]])
#Transfer blood to labelled eppendorf tubes.
 
#Let blood clot
 
#Centrifuge 20 min at 5000g (about 2000 RPM in eppendorf 5415C centrifuge)
[[Category:Animal Work]]
[[Category:Mouse Work]]
[[Category:Glucose]]

Latest revision as of 16:43, 10 February 2025

Materials

  • Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)

Protocol

  1. Collect serum (see Collecting_and_Storing_Mouse_Serum)