Deparaffinizing Histology Slides: Difference between revisions
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Materials | ==SOP== | ||
*[[SOP- Corrosives]] | |||
*[[SOP- Irritants]] | |||
*[[SOP- Flammables and Combustibles]] | |||
==Materials== | |||
#Xylene | #Xylene | ||
| Line 10: | Line 15: | ||
#Xylene: 2 x 3 minutes | #Xylene: 2 x 3 minutes | ||
#Xylene 1: | #Xylene 1:1 with 100% Ethanol: 3 minutes | ||
#100% Ethanol: 2 x 3 minutes | #100% Ethanol: 2 x 3 minutes | ||
#95% Ethanol: 3 minutes | #95% Ethanol: 3 minutes | ||
Latest revision as of 21:52, 4 June 2020
SOP
Materials
- Xylene
- 100% Ethanol
- 95% Ethanol
Method
- Use slide rack/holder
- Xylene: 2 x 3 minutes
- Xylene 1:1 with 100% Ethanol: 3 minutes
- 100% Ethanol: 2 x 3 minutes
- 95% Ethanol: 3 minutes
- 70% Ethanol: 3 minutes
- 50% Ethanol: 3 minutes
- Running cold tap water to rinse
Keep the slides in the tap water until ready to perform staining protocol.