Glucose Assay in Serum

From Bridges Lab Protocols
Revision as of 00:50, 13 February 2026 by Tiangebu (talk | contribs) (→Procedure)
Jump to navigationJump to search

Procedure

  • Plan out 96-well plate, including samples and space for 5 standards and a blank, in duplicates.
  • Add 100 uL of Color Solution to each well.
  • Add 0, 1, 2, 3, 4, 5 uL to each of the standard wells.
  • Add 3 uL of sample to each well.
  • Incubate at room temperature for 30 mins (or 5 mins at 37C).
  • Read plate at 505 nM and 600 nM.
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php?title=Glucose_Assay_in_Serum&oldid=2762"