TOPO Cloning: Revision history

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2 May 2009

  • curprev 00:4500:45, 2 May 2009 Davebridges talk contribs 611 bytes +611 Created page with '#PCR desired insert using Pfu polymerase. Add 1 uL of Taq and mix. Incubate 10min at 72C to add A to 3’ ends. Use immediately #If PCR product is a single band, no need to pu...'
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/TOPO_Cloning"