View source for MTM1 Genotyping

[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]
[[ Category:DNA]]
[[ Category:Molecular Biology]]

See http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153

==Primers==

*'''MTM1 Fwd''': GGC CCC ATT AAG GGA AGA GT
*'''MTM1 WT Rev''': GAT CTT CTG GGA AAG GCA CA
*'''MTM1 Mut Rev''': GCC AGA GGC CAC TTG TGT AG

==PCR Program==

* 94 for 2 min
* 10 cycles of:
** 94 for 20sec	
** 65 for 15sec	with -0.5 C per cycle
** 68 for 10sec	
* 28 cycles of:
** 94 for 15sec
** 60 for 15sec
** 72 for 10sec
* 72 for 1 min
* 4 hold

==Expected Results==

[[File : MTM1_Genotyping_Result.jpg‎]]