View source for Yeast Selective Media and Agar

==Materials==
*Minimal SD Base (Clontech 630411)
*Dropout Supplement (as an example -Leu DO supplement has all the nutrients '''except''' leucine)
*Bacto Agar (if making plates)

Selective yeast media is made so that a particular amino acid or nutrient (typically Leu, Trp, Ura, His or Ade) is missing and must be provided by the yeast strain.  Therefore a yeast strain which is auxotrophic for that nutrient can only grow on that selective media if a selective plasmid is provided.  See [[http://en.wikipedia.org/wiki/Auxotrophy Wikipedia Article on Auxotrophy]].  As an example, BY4742 is auxotrophic for Leu, Lys, His and Ura.  Therefore this strain cannot grow on -Leu plates, unless transformed with a plasmid containing the LEU2 gene.  In this way transformants can be selected.

==Preparation==
*Weigh out 2.67g/100 mL of Minimal SD Base and 0.069g/100mL of the appropriate dropout supplement.
*If making plates, add 2g/100mL of bacto-agar
*Stir to dissolve and check that pH is at 5.8
*Autoclave for 20 min at 121C using the liquid cycle
*Unless adding adenine hemisulfate, there is no need to let the media cool, so plates can be poured immediately.
*Pour plates, let dry and store in the cold room

[[Category:Buffers]]
[[Category:Media]]