View source for Rapamycin Sensitivity Assay of Yeast

==Materials==
*YPD Media
*YPD Plates with either DMSO or 2-200 nM Rapamycin
*Yeast strains, streaked out on appropriate plate (< 1 month old)

==Protocol==
#Innoculate 3 mL of YPD in a small erlenmeyer flask with one colony of yeast
#Grow for ~8h at 24C
#Check OD-600
#Estimate how much to dilute yeast so that the next morning the OD is 0.3.  For example, if the doubling time is 2.25h, then from 5 PM to 9 AM is 16h or 7.1 doublings, of 137 times the OD.  Therefore to get an OD of 0.3, you need to innoculate at 0.3/137 = 0.00219.
#For each flask innoculate several dilutions